Synthesis Scale

The synthesis scale refers to the nominal quantity (in moles) of the first (3’) nucleotide and not the final yield of the full length oligonucleotide. It is only approximate and some variation is to be expected. The delivered quantity will depend on the sequence length and purity. Minimum yields for a 20 base oligo are:

Minimum yields for a typical 20-mer oligo
Scale	Sequencing/PCR Grade	Transfection Grade	HPLC Purified
1 OD = ~30 ug = ~5 nmoles for a 20-mer For expected yields for longer or shorter oligos please refer to the FAQ. Click here for yields of Dual Labeled Probes
40 nmole	5 OD	4 OD	2 OD
200 nmole	20 OD	16 OD	8 OD
1000 nmole	80 OD	64 OD	32 OD

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