Service Options & Pricing

New simplified service structure - updated May 2010

GeneWorks recently expanded its services to include longer read length sequencing, higher guaranteed yields, and additional applications. Our service now offers:

Guaranteed turnaround and sequence quality
Guaranteed sequence yields per lane
Choice of read length – 35, 65 or 90b
Single or paired-end reads
Sample indexing for multiplexed sequencing
Customer references available

GeneWorks’ Service includes:

Advice and project scoping discussions for optimal results
Secure and easy sample pick up
Quality control of sample upon receipt
Data delivery

Also available

Sequence data analysis and genome assembly

Current Applications and Library Preparation Methods

Library for:	Basis of sample prep (done by GeneWorks)	Sample requirement
RNA-based Applications
Small RNA (eg miRNA) sequencing (using v1.5 Illumina protocol)	Ligation of 5' and 3' RNA adapters, RT, amplification	2 µg total or small RNA-enriched RNA
mRNA sequencing*	polyA purification, fragmentation, random-primed RT, amplification	10 µg total RNA or 100 ng polyA⁺ RNA
Other Applications		Please enquire
DNA-based Applications
DeepCAGE sequencing*	Enzymatic generation of tags from mRNA specific to Transcription Start Sites	50 µg total RNA
Other Applications		Please enquire
Customer-supplied Libraries
Array-captured eg Nimblegen DNA prepared to contain Illumina sequences*	Direct sequencing	100 ng DNA
Other customer-prepared libraries prepared to contain Illumina sequences*	Direct sequencing	100 ng DNA
NOTES: *Library format compatible with Multiplexed sequencing. For successful library preparation ChIP and MeDIP DNA must be quantified by PicoGreen assay or equivalent and contain sufficient DNA in the 200-400 bp range. If MeDIP DNA is prepared using anti-5-Me-dC antibody, adapter ligation by GeneWorks is required prior to the customer immunoprecipitation step.
DNA-based Applications
Whole Genome Sequencing (single-end or paired-end libraries)*	Fragmentation, adapter ligation and amplification	5 µg purified gDNA
Whole Genome Sequencing (Mate-pair libraries)*	Fragmentation (1-4 kb), biotin end labeling, circularisation, fragmentation, selection of biotinylated DNA, adapter ligation, amplification	10 µg purified gDNA
Targeted sequence capture on array using imaGenes service*	Array design, manufacture, enrichment, library preparation, QC	30 µg purified gDNA
Targeted sequence capture using Agilent SureSelect system	Library preparation, enrichment, amplification, QC	10 µg purified gDNA
Whole Human Exome capture using Nimblegen SeqCap EZ system	Library preparation, enrichment, amplification, QC	10 µg purified gDNA
Array-captured eg Nimblegen DNA prepared to contain with non-Illumina sequences*	Concatenation, fragmentation, adapter ligation and amplification	5 µg purified DNA
Pooled PCR products*	Fragmentation (or concatenation then fragmentation), adapter ligation and amplification	5 µg purified DNA
ChIP or nucleosomal DNA sequencing*	Adapter ligation and amplification	20 ng purified DNA¹
MeDIP sequencing*	Adapter ligation prior to MeDIP, amplification	20 ng purified DNA^1,2
Bisulphite DNA sequencing*	Fragmentation, ligation of methylated adapters, bisulphite conversion, amplification	Please enquire
Other applications		Please enquire

Sequencing Options

**SEQUENCING OF PREPARED LIBRARIES (per lane of Flow Cell)**
Minimum 15 Million Reads per Lane (30 Million for Paired-End) Guaranteed¹
Read Length	Single-end reads	Paired-end reads	With index read for multiplexed sequencing²
35 b	•
35 b	•		•
35 b		•
35 b		•	•
65 b	•
65 b	•		•
65 b		•
65 b		•	•
90 b	•
90 b	•		•
90 b		•
90 b		•	•
NOTES: Sequence guarantee assumes that satisfactory library quality is obtained from the sample. Up to 12 samples can be sequenced in one lane using the Illumina indexing system. Price includes separation of sequences into separate files by indexing sequence.

Pricing

To obtain a copy of our service Price List please email sequencing@geneworks.com.au.

Sub Navigation

Extended web ordering for GeneWorks Oligos!
More features - same convenience.

Page Navigation Anchors