EX-203400: SYBR® Green master mix, Universal RT, 25ml
miRCURY LNA™ Universal RT microRNA PCR, SYBR® Green master mix, 2500 rxns of 20µl or 5000 rxns of 10µl
miRCURY LNA™ Universal RT microRNA PCR, Polyadenylation and cDNA synthesis kit contains reagents for polyandenylation and reverse transcription of all microRNAs into cDNA in a single reaction step. By using the same RT reaction as template in all subsequent PCR reactions, the procedure is greatly simplified compared to systems that require microRNA-specific first-strand synthesis. The kit contains enough reagents for 32 20µl reverse transcription reactions. The following materials are supplied: 5x reaction buffer, 10x enzyme mix, nuclease free water and a synthetic RNA spike-in template. The SYBR® Green master mix contains 25mL of 2x SYBR® Green master mix, nuclease-free water and a Control primer set. The Control primer set is designed for amplification of the synthetic RNA spike-in provided in the Universal cDNA synthesis kit. NOTICE TO PURCHASER: LIMITED LICENSE Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser’s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. SYBR® Green is a trademark of Invitrogen.