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The scientists at Alvéole have developed an innovative multi-protein photopatterning technique (PRIMO), which involves creating quantitative protein patterns on which living cells are cultured. The PRIMO technique is based on LIMAP* technology (Light Induced Molecular Adsorption of Proteins) and combines a UV illumination system controlled by dedicated software (called “Leonardo”) and a specific photo-activatable reagent (PLPP). Working together, these two key system components make it possible to generate, in only a few seconds, any multi-protein pattern, including overlapping gradients, on standard cell culture substrates
This technology opens up new possibilities for studying the influence of the microenvironment on intracellular and intercellular mechanisms, which is an essential part of research in cell and medical biology, including such areas as stem cell research, and cell-based assays for drug development and predictive toxicology.
*“Multiprotein Printing by Light-Induced Molecular Adsorption” Strale P.O. et al, Adv Mater. 2015
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